Construct:Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To construct pBlueBac-human-ACC2, a ̃7.5 kb fragment of a Kpn I/Xho I double digestion of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University; SEQ ID NO: 5) was ligated with a pBlueBac4.5 vector digested with same set of restriction enzymes.
Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To identify the wild type human ACC2 amino acid sequences, the following three amino acid sequences were compared: 1) the sequence of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University), 2) the coding region of human ACC2 predicted by human genomic contig AC007637 (defined by 12q24 BAC RCPI11-443D10; Roswell Park Cancer Institute Human BAC Library, complete sequence), and 3) Genbank ACC2 sequence (NM—001093; Ha et al., (1996) Proc.
Plasmid Preparation:Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To construct pBlueBac-human-ACC2, a ̃7.5 kb fragment of a Kpn I/Xho I double digestion of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University; SEQ ID NO: 5) was ligated with a pBlueBac4.5 vector digested with same set of restriction enzymes.
Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To identify the wild type human ACC2 amino acid sequences, the following three amino acid sequences were compared: 1) the sequence of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University), 2) the coding region of human ACC2 predicted by human genomic contig AC007637 (defined by 12q24 BAC RCPI11-443D10; Roswell Park Cancer Institute Human BAC Library, complete sequence), and 3) Genbank ACC2 sequence (NM—001093; Ha et al., (1996) Proc.
Sequencing:Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To construct pBlueBac-human-ACC2, a ̃7.5 kb fragment of a Kpn I/Xho I double digestion of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University; SEQ ID NO: 5) was ligated with a pBlueBac4.5 vector digested with same set of restriction enzymes.
Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To identify the wild type human ACC2 amino acid sequences, the following three amino acid sequences were compared: 1) the sequence of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University), 2) the coding region of human ACC2 predicted by human genomic contig AC007637 (defined by 12q24 BAC RCPI11-443D10; Roswell Park Cancer Institute Human BAC Library, complete sequence), and 3) Genbank ACC2 sequence (NM—001093; Ha et al., (1996) Proc.
BAC Assay:Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To construct pBlueBac-human-ACC2, a ̃7.5 kb fragment of a Kpn I/Xho I double digestion of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University; SEQ ID NO: 5) was ligated with a pBlueBac4.5 vector digested with same set of restriction enzymes.
Article Title: Acetyl CoA carboxylase 2 sequences and methods
Article Snippet: To identify the wild type human ACC2 amino acid sequences, the following three amino acid sequences were compared: 1) the sequence of pYES-human-ACC2 (purchased from Dr. Ki-Han Kim, Purdue University), 2) the coding region of human ACC2 predicted by human genomic contig AC007637 (defined by 12q24 BAC RCPI11-443D10; Roswell Park Cancer Institute Human BAC Library, complete sequence), and 3) Genbank ACC2 sequence (NM—001093; Ha et al., (1996) Proc.
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